Astragaloside IV Inhibits Proliferation and Induces Apoptosis of Melanoma B16 Cells
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Abstract:
The effect of astragaloside IV on the proliferation and apoptosis of melanoma B16 cells was investigated. After the cells were cultured successfully, they were divided into blank control group, low concentration group (10 mg/mL), medium concentration group (20 mg/mL) and high concentration group (50 mg/mL). The effects of astragaloside IV on proliferation and apoptosis of melanoma B16 cells were evaluated, and the expression levels of caspase-3, B-cell lymphoma-2 (Bcl-2), bax and β-catenin were detected. The results showed that astragaloside IV had good antioxidant activity. When treated with 50 mg/mL, the reducing ability and DPPH free radical scavenging capacity were 0.92 and 70.82%, respectively. Under this concentration, the proliferation rate and apoptosis rate of B16 cells after 72 h treatment were 15.55% and 40.45%, respectively, which were significantly different from those of other groups (p<0.05). In addition, the expression levels of caspase-3, Bcl-2, bax and β-catenin were 0.56, 0.58, 0.27 and 0.25, respectively, which were significantly different from those of other groups (p<0.05). Results indicated that astragaloside IV could inhibit the proliferation of melanoma B16 cells and induce apoptosis by regulating the expression of caspase-3, Bcl-2, bax and β-catenin.
