Inhibition Effects of Loganin on Fetal Bovine Serum-induced Steatosis in Human Liver Cell Line L02
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Abstract:
In this research, 50% fetal bovine serum (FBS)-induced steatosis in human liver cell line L02 cell was used as model to investigate the therapeutic effects of loganin on cellular steatosis. The inhibitive effect of loganin on cell proliferation was detected with MTT method and Oil red O staining method. The enzymatic activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) and levels of total cholesterol (TC) and triglycerides (TG) in cell supernatant, as well as the intracellular levels of TC, TG and thiobarbituric acid reactive substances (TBARS) were determined with corresponding assay kits. MTT results showed that compared with the model group, different concentrations of loganin could significantly inhibit the growth of steatosis L02 cells (p<0.05), the inhibitory rate was 14.6%~26.91%. Oil red O staining showed that the accumulation of hepatic lipid in the loganin treatment groups was significantly decreased with the increase in concentration. Compared with the model group, the extracellular enzyme activities of AST, ALT and LDH in loganin treatment groups were reduced by 26.70%~52.58%, 24.83%~61.23% and 14.53%~28.83%, respectively; extracellular levels of TC and TG were reduced by 50.46%~68.76% and 32.51%~53.37%, respectively; intracellular levels of TC and TG were reduced by 11.48%~32.79% and 10.50%~18.30%, respectively; intracellular levels of TBARS were reduced by 40.33%~60.10%. These inhibitory effect of loganin treatment groups on cellular steatosis was dose-dependent and better than that of spontaneous recovery group. The results indicated that loganin could inhibit FBS-induced cellular steatosis in L02 cells via effectively reducing lipid metabolic disorders and lipid peroxidation in L02 cells.
