Improvement of Rheumatoid Arthritis with Lactobacillus plantarum HG20 in Rats
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Abstract:
In order to obtain a new strain of lactic acid bacteria with adjuvant therapy of rheumatoid arthritis, morphological observation, API50 CHL sugar fermentation experiment, 16S rDNA and pheS gene sequence comparison were performed to isolate a strain HG20 from the surface of cucumber for further identification. The therapeutic effect of the HG20 strain on rheumatoid arthritis model rats induced by type II collagen was evaluated according to the indices including the degree of ankle swelling, levels of serum inflammatory factor, and rheumatoid-related indicators. The results showed that HG20 was identified as a Lactobacillus plantarum strain. Animal experiment results showed that after 21 days of administration, the ankle circumference of rats in the HG20 group decreased from 3.98 cm to 1.31 cm (by 65.52%), and the toe volume decreased from 6.61 mL to 2.35 mL (by 61.28%).Compared with the model group, L. plantarum HG20 could significantly reduce the levels of serum inflammatory cytokines (IL-1β by 38.26%; TNF-α by 52.58%), increase the expression of anti-inflammatory factor (IL-10 by 55.38%),reduce C-response protein (CRP by 12.89%) and immunoglobulin (IgG by 46.07%; IgM by 34.29%). In summary, L. plantarum HG20 has adjuvant therapeutic effects on rheumatoid arthritis through reducing inflammatory response and regulating autoimmunity. It is a new probiotic strain with potential application prospects.
