Optimization of Extraction Process and Component Analysis of the Flavonoids from Broussonetia papyrifera Leaves
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Abstract:
In order to investigate the optimal extraction process and the main components of flavonoids from the leaves of Broussonetia papyrifera, single factor experiments and response surface methodology (RSM) analysis were conducted to examine the effects of ethanol concentration, extraction time, extraction temperature and liquid-material ratio on the yield of flavonoids, as well as the flavonoid composition of the extract. The results showed that when the ethanol concentration was 30%, extraction time was 2.5 h, the extraction temperature was 69 ℃, and liquid-material ratio was 9%, the yield of flavonoids in Broussonetia papyrifera leaves was 37.33 mg/g; UPLC-MS (UPLC-MS/MS) was used for component analysis, a total of 11 species of flavonoids were identified: scutellarin, orientin, apigenin-7-O-glucuronide, kaempferol, apigenin, cynaroside, galangin, apigenin-7-glucoside, (1ξ)-1,5-anhydro-1-(5,7-dihydroxy-4-oxo-2-phenyl-4h-chromen-8-yl)-d-glucitol, 6-O-phydroxybenzoylaucubin, and isovitexin. The Box-Behnken Design of response surface methodology was used to optimize the ethanolic extraction of total flavonoids form Broussonetia papyrifera leaves, while determiningthe best extraction process for the total flavonoids. The process was simple and easy to implementwith good stability, which can provide guidance and +referencefor the industrial production of flavonoids from Broussonetia papyrifera leaves.
