Optimization of Spore Germination Conditions of Clostridium sporogenes
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Abstract:
To study the optimum conditions for the spore germination of Clostridium sporogenes under different temperature, time and pH values,in this study, the spore suspension of Clostridium sporogenes was prepared by activating the passaged Clostridium sporogenes solution for 7 days, centrifugation for several times, and adding sterile water. The treatment temperature was 70 ℃~90 ℃, and the treatment time was 5~25 ℃. The germination rate of Clostridium sporogenes was observed and calculated by plate counting method, and the optimal values of three conditions affecting the germination rate of Clostridium sporogenes were studied by response surface methodology. Through the analysis of the quadratic multiple regression equation, the best conditions for spore germination of Clostridium sporogeneswere obtained as follows: when the temperature was 80.90 ℃, the time was 20.72 min, and the pH value was 4.69, the highest germination rate of Clostridium sporogenum could reach 80%. In this paper, combined with the mechanism of spore germination, the optimum germination conditions of spores were used to make the spores germinate under mild conditions, which laid the foundation for the new sterilization method of "germination first and then killing"; and provided a new idea and feasibility of low-intensity sterilization for the difficult problem of spore killing, which was of great significance to the application of low-intensity sterilization in the sterilization of low acid canned food.
