Analysis of Chemical Components of Perilla frutescens Based on Ultra High Performance Liquid Chromatography-Quadrupole / Time of Flight Mass Spectrom (UHPLC-Q/TOF MS)
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Abstract:
UHPLC-Q/TOF MS was used to qualitatively analyze the chemical components of Perilla frutescens treated by shade-drying menthod or freeze-drying method, and the material basis of the differences between the two treatments from the same source was discussed. High performance liquid chromatography (HPLC) conditions were as follows: Welch Ultimate UHPLC C18 column (2.1mm X100mm, 1.8 μm), the mobile phase gradient elution with 0.1% formic acid water solution and acetonitrile. The mass spectrometry conditions were as follows: AB Sci ex Triple TOF 5600 high resolution tandem mass spectrometry, electrospray ionization (ESI) ion source, data collection in positive and negative mode, fragmentation by retention time, accurate molecular ion peak, and two stage mass spectrometry. A total of 51 compounds were detected in Perilla frutescens, 45 compounds in shade-drying Perilla frutescens, 35 compounds in freeze-drying Perilla frutescen (including flavonoids, styrene acrylic element class, organic acids, etc.). There are nine compounds (four organic acids, four flavonoids, one element of styrene acrylic) was the first identified in Perilla. The composition could be changed after Perilla treated with the shade-drying or freeze-drying. The chemical components were different between Perilla treated with shade-drying method and Perilla treated with freeze-drying method. The results were helpful to choose different treatment methods of Perilla for certain medicinal value. It could be also helpful in further evaluation for Perilla, and their medicinal substances in the body.
