Evaluation of Antioxidant and Immunomodulatory Effects of Radix codonopsis-Poria cocos- Radix glycyrrhizae Water Extract in Mice with Learning and Memory Impairment
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Abstract:
To study the effects of traditional Chinese medicine Radix codonopsis, poria cocos and Radix glycyrrhizae water extract on learning and memory impaired mice, the antioxidant and immunomodulatory effects of water extract on learning and memory impaired mice and normal mice were evaluated. Intraperitoneal injection of scopolamine hydrobromide was used to model learning and memory impairment. ELISA was used to detect SOD, GSH-Px enzyme activity and MDA content in hippocampus of mice, serum IL-2, TNF-α level, and spleen NK cell activity. The anti-fatigue effect of water extract on normal mice was evaluated in weight-bearing swimming experiment. Thymus index and spleen index were determined by gravity method. The results showed that the high dose of water extract (40 g/kg·d) had the best effect, and the novel object recognition index of memory impaired mice could be increased by 18.35% without affecting the independent activity of mice. SOD and GSH-Px increased by 65.56 U/mg, 437.00 U/mg, and MDA decreased by 6.30 nmol/mg. IL-2 and TNF-α decreased by 78.43 pg/mL, 160.20 pg/mL, and the activity of NK cell increased by 2488.81 U/g. It was also possible to increase the weight swimming time of normal mice by 60.12 s compared with the normal group. Thymus index and spleen index increased by 0.06% and 0.10%; SOD and GSH-Px increased by 79.52 U/mg and 345.30 U/mg, respectively, MDA decreased by 1.05 nmol/mg, and NK cell activity increased by 1816.31 U/g. The results showed that the water extract of Radix Codonopsis-Poria cocos-Glycyrrhizae Radix could improve the learning and memory impairment of mice and exert anti-fatigue effect on normal mice, its mechanism was related to its anti-oxidation and immunomodulatory function.
