Abstract:In this work, the Ag nanoparticles / gliadin / chitosan composite antimicrobial films were successfully constructed using the cast method to achieve the stabilization and synergistic effect of Ag nanoparticles (AgNPs). Self-assembled gliadin colloidal particles acted as both chelator and stabilizer of AgNPs, effectively inhibited the aggregation of AgNPs. As compared with other films, gliadin /AgNPs composite films possessed excellent transparency. SEM and EDS confirmed that AgNPs were evenly distributed on the composite film. Moreover, gliadin /AgNPs composite films had excellent sustained-release properties. Human skin fibroblast evaluation results showed that the composite films have better biocompatibility. More important, confocal laser scanning microscope (CLSM) revealed that the gliadin /AgNPs composite films possessed stronger antibacterial effect on E. coli and S. aureus. These films are of great value in the development of antibacterial food packaging materials, wound dressings and implants.
