Determination of Mercury Speciation in Aquatic Animals and Their Products by LC-hydride Generation-ICP
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Abstract:
A simple and efficient method for the determination of methylmercury (MetHg) , Ethyl Mercury (EtHg) and inorganic mercury (Hg2+) in aquatic animals and their products was developed by combining Inductively coupled plasma with High-performance liquid chromatography hydride generation. Microwave acid extraction was used to pretreat the sample, optimize the chromatographic conditions and carrier concentration, and then verify the method's precision, detection limit, accuracy and standard recovery. The results showed that when the methanol content in the mobile phase was 5% and the l-cysteine content was 0.10%, the separation effect, the peak type and the instrument response value were good, and the Mercury memory effect was basically eliminated. The separation of inorganic mercury, methyl mercury and ethyl mercury was realized within 7 minutes. The linear relationship was good, r>0.9990, RSD%<10.00%. The quantitative limits (LOQ) were 0.023, 0.032 and 0.038 mg/kg, respectively. The recovery rate was 82.50%~98.40 %. The accuracy of the method was verified by two aquatic animal reference materials, BCR463 and TORT-3. The method is simple, effective, accurate and suitable for the determination of mercury species in aquatic animals and their products. It provides a new method for mercury speciation analysis.
