Determination of 78 Chemical Drugs Illegally Added in Health Food by Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry
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Abstract:
A method was developed for the determination of 78 chemical drugs illegally added in three types of health foods (slimming, anti-fatigue and sexual promoting health foods) by QuEChERS--ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The samples were extracted with methanol. The extracted solution was purified with QuEChERS approach. The seventy eight chemical drugs were then separated on a ACQUITY UPLC HSS T3 column (2.1×100 mm, 1.7 μm) through gradient elution with acetonitrile and 10 mmol/L of ammonium acetatesolution (0.1% formic acid) using as mobile phase and detected by MS/MS under multi-reactions monitoring (MRM) modes (ESI+ and ESI-)). The chemical drugswere quantified with external standard method. Under the optimal conditions, these chemical drugs were well separated, and had good linear relationship (r≥0.9991). The limits of detection (LOD) (S/N≥3) were 0.5 μg/kg~420 μg/kg. The average recoveries at three spiked levels were in the range of 59.9%~120.7%, with the relative standard deviation (RSD, n=3) of 1.3%~16.2%. This newly developed method was successfully applied for the determination of 57 health food samples, in which 14 chemical drugs were found in 41 samples. The developed method is rapid, simple, sensitive and highly efficient which can be used for the qualitative and quantitative determination of illegally added chemical drugs in the slimming, anti-fatigue and sexual promoting health foods.
