Optimization of Gallic Acid Production by Enzymatic Hydrolysis of Tara Tannin
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Abstract:
Gallic acid had wide application in food, chemistry and pharmaceutical industries. Compared with the traditional chemical production approach, the enzymatic hydrolysis had the advantages of environmental friendness and fewer by-product. In this work, a previously developed thermal stable tannase Tan1 was used for the simultaneous leaching-enzymolysis of Tara tannin and the hydrolysis conditions were optimized for the high efficiency enzymatic production of gallic acid. First, the preliminary hydrolysis conditions were optimized by single factor test as followed: Tara powder liquid ratio 1:30, reaction temperature 60 ℃, enzyme dosage 4 mL and reaction pH=5. Based on the single factor test, the Plackett-Burman design was performed to investigate the effects of different factors on the reaction conditions of tannase hydrolysis of Tara powder. It was found that temperature, pH and ratio of material to liquid were the significant factors that affect the hydrolysis process. Furthermore, the optimal hydrolysis conditions were optimized by response surface design as follows: Tara powder liquid ratio 1:40, reaction temperature 65 ℃, pH=4, the yield of gallic acid was 65.89%. The continuous feeding reaction was also investigated under the optimal hydrolysis conditions. The results indicated that Tara tannin could be hydrolyzed into gallic acid with high efficiency during six batch feeding process. This research laid the foundation for the industrialization of gallic acid produced by enzymatic hydrolysis of Tara tannin.
