Alleviating Action of Sodium Selenite on Immunological Stress in Ictalurus punctatus during Temporary Cultivation
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Abstract:
To determine the effect of sodium selenite (Na2SeO3) on blood indexes of Ictalurus punctatus during the process of temporary cultivation, in this study, the Ictalurus punctatus were randomly divided into four groups (n=20 with fish water ratio of 130 g: 1 L in each group), the Na2SeO3 was added to the water, and the initial concentrations were 0 (control group), 0.1, 0.2, 0.5 mg/L, respectively. Temporary cultivation for 4 days with fasting, the related serum biochemical indexes such as liver metabolism, energy metabolism, immune protein and oxidative stress were detected every day. The results showed that, at the initial stage of cultivation, the alanine aminotransferase (ALT) and glutamic oxaloacetic transaminase (AST) in serum of 0.1 mg/L Na2SeO3 group were significantly lower than that of 0.2 mg/L group and 0.5 mg/L group (p<0.05). With the increase of cultivation days, ALT and AST activities in serum in the Na2SeO3 groups were decreased. And the activities of ALT and AST in serum of 0.1 mg/L Na2SeO3 group were significantly reduced in the 2 days of temporary cultivation (p<0.05) compared with the control group, amounted to 75.39% and 170.13% respectively. With the prolongation of cultivation time, the concentration of MDA in the 0.1 mg/L Na2SeO3 group was significantly lower than that in the 0.2 mg/L and 0.5m/L groups. Moreover, the blood glucose in the 0.1 mg/L Na2SeO3 group increased significantly (p<0.05) at first and then decreased significantly (p<0.05) with the increase of cultivation time, which was contrary to the trend of the control group, delaying the rate of blood glucose decline. The above results showed that when the fish water ratio was 130 g:1 L, the initial concentration of Na2SeO3 in water was 0.1 mg / L for about 2 days, which could alleviate the stress response, delay the process of blood glucose reduction caused by fasting, and improve the antioxidant capacity of Ictalurus punctatus.
