Evaluation of Antioxidant and Anti-inflammatory Activities and Analysis of Chemical Components in Different Extraction Parts of Fresh Longya Lilium
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Abstract:
In this paper, the oxidative stress model induced by H2O2 and the inflammatory model induced by lipopolysaccharide (LPS) were used to evaluate the antioxidant and anti-inflammatory activities of the extracts from 80% ethanol solution of fresh Longya Lilium, and the chemical components of the extraction parts with significant activities were analyzed by ultra-high pressure liquid chromatography tandem quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS).The results showed that the water saturated n-butanol extract part(50 μg/mL) had the strongest antioxidant capacity, which increased the activity of antioxidant enzymes in RIN cells by 2.78 times (SOD), 3.79 times (GSH), 6.27 times (CAT); the chloroform extract part (50 μg/mL) had the strongest anti-inflammatory activity, and the inhibition rates of NO, TNF-α, IL-6 in RAW 264.7 cells were 55.21%, 20.86% and 84.95% respectively. In addition, the antioxidant activity of water saturated n-butanol extraction part was stronger than that of chloroform, and the anti-inflammatory activity of chloroform extraction part was stronger than that of water saturated n-butanol. The water saturated n-butanol part is mainly composed of 12 compounds (including 1 lignan, 5 phenylpropanoic acids and 6 steroidal saponins), the chloroform part is mainly composed of 14 compounds (including 7 phenylpropanoic acids, 1 diosgenin and 6 steroidal saponins), and 4 compounds are common components. Therefore, phenylpropanoic acid and steroidal saponins may be the material basis of fresh Longya Lilium's antioxidant and anti-inflammatory activities, and most of them are concentrated in the extraction parts of water saturated n-butanol and chloroform. These results provide theoretical reference for further elucidating the material basis of Lilium's biological activities and its development and utilization.
