Polysaccharides Extract from Codonopisis pilosula Enhances the Vitality and Antioxidant Activity of Drosophila melanogaster
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Abstract:
In order to evaluate the effect of Codonopisis pilosula polysaccharides on the reproduction capability and life span and antioxidant capacity of Drosophila melanogaster, survival experiments were conducted to examine the influences of different concentrations (50, 100, 200, 400 mg/L) of Codonopisis pilosula polysaccharides on the reproduction capability and life span of four generations (F1 to F4), the number of female and male, the survival time in paraquat treatment, and the activity of antioxidant enzyme superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) contents. The results showed that the 400 mg/L dose of CPP could significantly increase the fertility of F1~F4 generations female and male Drosophila generations. Compared with the control, the number of offspring Drosophila generations increased by 150% (p<0.01), and extended the life span of F1 to F4 female and male Drosophila generations. The lifespan of offspring Drosophila generations increased by 34.07% compared with the control (p<0.01). Compared with the control, the survival time of F1~F4 generations female and male fruit flies under the acute oxidative damage of paraquat were also prolonged (7.06 and 6.28 h (p<0.01), respectively). At the same time, the activities of SOD and CAT in Drosophila generations increased (p<0.01), and the content of MDA was reduced (p<0.01). It indicated that high dose of Codonopisis pilosula polysaccharides could increase the reproduction capability and extend the life span of F1 to F4 female and male Drosophila generations. After treated with the high dose of Codonopisis pilosula polysaccharides, the survival time in paraquat treatment was improved, the contents of MDA in the Drosophila generations was reduced and the activity of SOD and CAT or the antioxidant capacity increased rapidly, exhibiting a dosage effect., and the sex ratio of female and male Drosophila generations maintain 1:1.
