Inhibitory Effect of Dihydromyricetin on the Proliferation of Osteosarcoma Cells
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Abstract:
In the present study, the inhibitory effect and mechanism of action of dihydromyricetin (DHM) on the proliferation of osteosarcoma cells were investigated. The inhibitory effects of DHM solutions at different concentrations on the proliferation of osteosarcoma cells were examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The influences of DHM solutions at different concentrations on the cell morphology, apoptosis and cell cycle of osteosarcoma cells were examined by an inverted microscope, Hoechst 33258 fluorescent staining and flow cytometry. The expression levels of cell cycle- and apoptosis-related proteins were analyzed by Western Blot method. The results showed that DHM exhibited a significant inhibitory effect on the proliferation of osteosarcoma cells and caused changes in the cell morphology of osteosarcoma cells in a dose-dependent manner, with the half inhibitory concentration (IC50) value as 24.41±1.25 μM. The results of cell cycle experiments showed that after the DHM treatment, the percentage of the cells at G0 phase to the cells at G1 phase (G0/G1) decreased significantly from 60.20% to 21.50%, whereas, the percentage of the cells at G2 phase to the cells at M phase increased from 11.60% to 45.30%, and the expression of cell cycle-related protein, Cyclin B1, decreased, causing the cell cycle arrest. In addition, the ratio of the expression of anti-apoptotic protein, Bcl-2, to the expression of pro-apoptotic protein, Bax, decreased, inducing apoptosis. These findings indicate that DHM has significant anti-proliferation activity against osteosarcoma cells, and may be a potential functional food or drug for the treatment of osteosarcoma.
