Study on the Effect of High γ-aminobutyric Acid-enriched Yoghurt on Sleep and Its Mechanism on Insomnia in Mice
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Abstract:
The effect of high γ-aminobutyric acid-enriched yoghurt on sleep and its mechanism on insomnia was accessed by male ICR mice. Hypnosis test of pentobarbital sodium subthreshold, test of extended pentobarbital sodium sleep time and barbital sodium sleep latency test were conducted to determine the effect of high γ-aminobutyric acid-enriched yoghurt on sleep. PCPA insomnia model was used to explore the influence of high γ-aminobutyric acid-enriched yoghurt on insomnia. The changes of Gly, GABA, 5-HT and Glu in mice brain tissue and serum were measured to determine the mechanism. Compared with control group, 80 and 160 mg/kg high γ-aminobutyric acid-enriched yoghurt could improve 60% sleep rate of mice (p<0.05). 80 mg/kg high γ-aminobutyric acid-enriched yoghurt could extent mice sleep time to 156.20 min (p<0.05). 40, 80 and 160 mg/kg high γ-aminobutyric acid-enriched yoghurt did not affect mice sleep latency. In insomnia experiment, high γ-aminobutyric acid-enriched yoghurt increased the content of GABA and Gly in the brain tissue to 1.10 ng/mL and 28.78 ng/mL, respectively. In conclusion, high γ-aminobutyric acid-enriched yoghurt could improve sleep mainly by affecting the content of GABA and Gly in mice brain.
