Improvement Effects of Moringa oleracea Seed Oil on Lipopolysaccharide-induced Intestinal Inflammation in Mice
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Abstract:
To investigate the effect and mechanism of Moringa oleracea seed oil on LPS-induced intestinal inflammation in male ICR mice, lipopolysaccharide (LPS, 3.5 mg/kg) was injected intraperitoneally to establish the intestinal inflammation model of mice. After 7 days, Moringa oleracea seed oil was administrated by gavage, and corn oil was used as the control. Changes in the body weight and colon tissue of mice were recorded. The levels of cytokines, includingIL-1β, IL-6, IL-18 and TNF-αin serum and colon were measured by ELISA assay, respectively. The mRNA levels of Nlrp3, Asc, Caspase-1 and IL-1β in colon were detected by qRT-PCR assay. Moringa oleracea seed oil significantly inhibited the weight loss and reduced the ratio of colon weight to length in LPS-treated mice (p<0.05). At the same time, the levels of IL-1β (175.44 ng/L and 184.12 ng/L), IL-6(82.12 ng/L and 58.19 ng/L), IL-18(82.91 ng/L and 52.32 ng/L) and TNF-α (51.75 ng/L and 81.31 ng/L) in serum and colon of the model mice were significantly reduced by the intervention of Moringa oleracea seed oil. The level of MPO in colon of model mice was significantly reduced to 86.32 ng/L (vs injury group: 113.59 ng/L, p<0.05). Compared with the injured group, Moringa oleracea seed oil inhibited the mRNA expression of Nlrp3 (39.31%), Asc (42.81%), Caspase-1 (35.59%) and IL-1β (28.62%). These results suggestted that Moringa oleracea seed oil could improve the intestinal inflammation in LPS-treated mice. The mechanism might be due to the reducing the activation of NLRP3 by Moringa oleracea seed oil in LPS-treated mice.
