Extraction Process of Polysaccharides from Lanzhou Lily Cores and Evaluation of their Antioxidant Activity in vitro
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Abstract:
The Lanzhou lily core in Gansu Province, China was used as the raw material for this study. According to the DB62/T 412 standard for grading, the core (lily slice width ≤14.90 mm) was extracted from the lily. The technological conditions for isolating polysaccharides from lily cores by hot water extraction were studied, and the antioxidant activity was compared and analyzed. Based on the single-factor test, the material-liquid ratio, extraction time, and extraction temperature were selected for the three-factor three-level Box-Benhnken test. The experiment data were analyzed using Design-Expert 11.0 software to obtain the best extraction conditions, and the crude polysaccharides that were extracted under the best conditions were evaluated for antioxidant activity. The results showed that the best lily core polysaccharide extraction conditions were a material-liquid ratio of 1:11.50, an extraction temperature of 69.18 ℃, and an extraction time of 53.82 min. Under these conditions, the polysaccharide yield was 10.30%, and the response surface test verification result was 10.29%. The relative error was 0.09%, which was relatively small. Lily core polysaccharides had different antioxidant activities in different antioxidant systems. Specifically, lily core polysaccharides had the best antioxidant activity at a concentration of 1.0 mg/mL, with an OH scavenging rate of 65.00% and a DPPH scavenging rate of 58.00%. However, the reducing power of lily core polysaccharides was weak and much lower than that of Vc at the same concentration. Therefore, the Box-Benhnken response surface method can be used in the lily core polysaccharide extraction process, and the optimization effect is good. At the same time, lily core polysaccharides have certain antioxidant activities, and the extraction of polysaccharides can be applied to lily cores in order to maximize the utilization of lily resources
