Isolation and Identification of Predominant Decaying Molds from Dried Betelnut during Storage
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Abstract:
The quality of dried betelnut during the storage was directly affected by microbial contamination. The separation and identification of predominant decaying molds were key steps in the safe storage of dried betelnut. In order to determine the species of dominant decaying molds in the storage of betelnut, 11 molds were isolated and purified from 5 batches of betelnut produced in Wanning, Hainan after 3 months of storage under high temperature and high humidity. Two mold strains were verified as preponderant decaying molds through the return infection experiments. By traditional morphological characteristics and microscopic structure observation, a preliminary identification was obtained. The ITS regions of the strains were amplified by polymerase chain reaction (PCR). The ITS gene sequences of 529 bp and 571 bp in length were obtained. Through comparison and analysis of those sequences, the phylogenetic trees were constructed. The strains were identified as Aspergillus glaucus and Aspergillus awamori, respectively. It provided a simple and reliable method for quick identification of spoilage mold in areca nut, and laid a good foundation for the optimization of storage conditions and the choice of the method for dried betelnut sterilization.
