Protective Effects of Lentinan on Bone Metabolism in Vitamin D Deficient Rats via Vitamin D-fibroblast Growth Factor-23-Klotho Axis
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Abstract:
To investigate the effect of Lentinan on bone metabolism in vitamin D deficient rats via vitamin D-fibroblast growth factor-23-Klotho axis, fifty SD healthy rats were selected, with 10 as control group, and the remaining 40 as the vitamin D deficient models (which were divided into the model group, vitamin D3 group (1000 U/kg), low-dose lentinan group (150 mg/kg) and high-dose lentinan group (450 mg/kg), with 10 rats per each group). The levels of serum calcium, phosphorus, alkaline phosphatase, vitamin D-fibroblast growth factor (FGF)-23-Klotho axis factors and bone metabolism indices were measured. The results showed that the serum levels of 25 (OH) VD3, 1,25 (OH) VD3, FGF-23 and Klotho in the control group were 75.58 ng/mL, 4.98 ng/mL, 468.25 pg/L, 62.58 pg/mL, respectively, while for the model group, the 25(OH)VD3, 1,25(OH)VD3 and Klotho decreased to 34.35 ng/mL, 3.52 ng/mL and 40.12 pg/mL, respectively, and the FGF-23 level increased to 896.35 pg/L. The differences between the data of these two groups of rats were statistically significant (p<0.05). The levels of 25 (OH) VD3, 1, 25 (OH) VD3 and Klotho in the high dose lentinan group increased to 71.24 ng/mL, 4.85 ng/mL and 58.68 pg/mL, respectively, which were statistically higher than those in the vitamin D3 group and the low dose lentinan group. Whereas, the level of FGF-23 of the high dose lentinan group decreased to 552.23 pg/L, which was statistically lower than those in the vitamin D3 group and the low dose lentinan group (p<0.05). Lentinan polysaccharides acts on the vitamin D-FGF-23-Klotho axis, regulates the axis factors, and increases the vitamin D content to improve osteogenesis and protect the bones of the vitamin D deficient rats.
