Modification of Rabbit Skin Gelatin Film Based on Burdock Extract
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Abstract:
In order to study the modification effect of Arctium lappa extract on rabbit skin gelatin membrane, Arctium lappa extract / rabbit skin gelatin composite membrane with the volume concentration of 0, 2%, 4%, 6%, 8% was prepared. The physical properties, antioxidative and bacteriostatic properties of each composite membrane were determined and its structure was characterized. The results showed that with the increase of burdock extract concentration, the water vapor filtration and water solubility of the composite membrane decreased gradually. When the concentration was 8%, the lowest values were 2.22×10-8 g/(m·s·Pa) and 19.22%, which were 14.94% and 27.56% lower than those of the control group, respectively. The tensile strength and thickness increased, and the maximum values were 67.11 MPa and 74.16 μ m, increased by 21.66% and 12.14% respectively, compared with the control group. The elongation at break first increased and then decreased, the concentration was 4% and the maximum was 4.86%, which was 63.63% higher than the control group. The light transmittance of the composite membrane was significantly reduced, and the light transmittance of the ultraviolet was significantly reduced, at the wavelength of 400 nm, the light absorption was the strongest, the transmittance was 0.003%, and the haze was significantly increased, the maximum was 37.59%. The clearance rate of hydroxyl radical of composite membrane was significantly increased, the maximum was 63.53%. The antibacterial ability of composite membrane was gradually enhanced, and the antibacterial abilities of composite membrane to different bacteria were ordered as: Bacillus subtilis > Staphylococcus aureus > Enterobacteriaceae > Aspergillus niger. The inhibition ability of Aspergillus niger increased gradually with the increase of the concentration of Arctium lappa extract, and the hydrogen bond formation and crystal formation of the composite membrane were found by infrared spectrum and scanning electron microscope analysis. Burdock extract promoted the unfolding and stretching of gelatin peptide chains. In summary, burdock extract can effectively improve the performance of rabbit skin gelatin film
