Enhancing Astaxanthin Accumulation in Chromochloris zofingiensis under Different Induction Conditions
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Abstract:
Chromochloris zofingiensis can grow in a variety of vegetative growth modes and synthesize astaxanthin under inductionconditions, and thus, is regarded as a promising and new alternative producer for microalgae-derived natural astaxanthin. The present studysystematically investigated the effects of sodium acetate concentration, feeding method, pH, and ethanol concentrations on the accumulation ofastaxanthin in C. zofingiensis, and the optimized conditions were then applied to an outdoor 300 L tubular photobioreactor for large-scaleverification. The results demonstrated that when the initial sodium acetate concentration was at 2.5 g/L, sodium acetate at 2.5 g/L was addedevery 6 days, medium pH was 6.5~8.3 and 2% ethanol was added, the astaxanthin content was the highest (3.71 mg/g), which increased by 31.71% compared to that achieved before optimization, and the corresponding astaxanthin yield of astaxanthin reached up to 5.37 mg/L. In the 300 L tubularphotobioreactor, the maximum astaxanthin content and yield were 3.44 mg/g and 1.10 mg/L, respectively. The present workenabled the enhancement on the accumulation of intracellular astaxanthin in C. zofingiensis using different induction conditions, and furtherverified the feasibility of cultivating C. zofingiensis in outdoor tubular photobioreactors for astaxanthin production.
