Determination of Magnolol and Honokiol from Magnolia officinalis Rehd. et Wils by HPLC-MS
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Abstract:
Microwave-assisted ionic liquid homogeneous liquid-liquid microextraction combined with high performance liquid chromatography tandem mass spectrometry was established to extract magnolol and honokiol from Magnolia officinalis Rehd. et Wils. In this study, ionic liquid was used as the extraction solvent. After microwave-assisted extraction, the ion-pairing reagent, ammonium hexafluorophosphate (NH4PF6), was added to the obtained sample extract, and the target analytes were separated and enriched by homogeneous liquid-liquid microextraction, and analyzed qualitatively and quantitatively by high performance liquid chromatography-tandem mass spectrometry. In this study, the conditions affecting the extraction efficiency were optimized by single factor and response surface experiments. The experimental results showed a good linear relationship (R>0.9999) for the target analytes within the detection concentration range, with the limit of detection (LOD) and limit of quantitation (LOQ) being 17.2~23.9 ng/mL and 57.4~79.6 ng/mL, respectively, and the spiked recovery between 89.25% and 95.00%. Compared with the traditional ultrasonic extraction and reflux extraction methods, the present method has advantages in the amount of solvent used and extraction time, and can be used for the extraction of magnolol and honokiol in Chinese herbal medicine and related pharmaceutical products.
