Arsenic Species Analyses of Mice Brain under Chronic Arsenic Exposure through Food
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Abstract:
In order to explore the effects of chronic arsenic (As) exposure in food on organisms, in this study, As-treated feeds with the proportion of the arsenic composition in rice were prepared and three arsenic-containing dose groups (Simulate dose group: 0.91 mg/kg, Low dose group: 9.10 mg/kg, High dose group: 30 mg/kg) were designed according to the dose gradient, which were used to feed C57BL/6 mice over three months and the contents of total arsenic and five arsenic species (iAsIII, iAsV, MMA, DMA and AsB) in blood and brain tissues of mice were determined. In groups of C, S, L and H, the total arsenic (tAs) contents in blood were 0.022, 0.023, 0.068 and 0.132 mg/kg, respectively, and the total arsenic contents in brain were 0, 0.006, 0.075 and 0.172 mg/kg, respectively. While the five arsenic species can be detected both in blood, and only DMA and some unknown As species (uAs) were detected in the brain in groups of L and H. The results presented a clear dose-related accumulations of total arsenic (tAs) reflected both in blood and brain, the detoxification process of arsenic in vivo also accelerated with the increase of exposure dose, and the ratio of organic arsenic/inorganic arsenic (oAs/iAs) can reflect the dose of arsenic exposure. Thus, the current study indicated that although the toxic iAs in food could not transfer across the blood-brain barrier and the toxicity of As in food especially for the staple should be concerned.
