Isolation, Identification and Pathogenicity Analysis of Vibrio parahaemolyticus Strains from Maowei Sea, Beibu Gulf
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Abstract:
To analyze the diversity characteristics of V. parahaemolyticus and safety risks in sea water and aquatic products in Beibu gulf, we collected samples from seawater and aquatic economic animals in the mariculture area of Maowei sea in Beibu gulf in this study. Marine vibrio was isolated from the samples by thiosulfate citrate bile salts sucrose agar culture medium (TCBS). Totally, 109 suspected vibrio strains were obtained. A total of 20 strains of V. parahaemolyticus were identified by DNA sequencing of 16S rDNA genes and specific function gene toxR after PCR amplification. Virulence gene tdh analysis showed that 4 of the 20 strains of V. parahaemolyticus carried the virulence gene tdh, with a detection rate of 20%. In addition, phylogenetic analysis also revealed that horizontal gene transfer happened in both the toxR and tdh gene sequences of V. parahaemolyticus. The results of this study indicated that V. parahaemolyticus in Beibu gulf had high genetic diversity, and some of them carry pathogenic factors, which is a risk of outbreak of aquaculture diseases and food-borne diseases in this area. Therefore, it is suggested to use PCR technology to detect specific species genes and virulence genes of V. parahaemolyticus, accurately evaluate the health safety in seawater and aquatic products in the Beibu gulf region, and reduce the risk of outbreak of aquaculture disease and food-borne diseases.
