The Antiproliferative Activities in Ganpu Tea on HepG2 and SGC-7901 Tumor Cell Lines
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Abstract:
In this study, the changes of components in the processed ganpu tea and their antiproliferative effects on HepG2 and SGC-7901 cells were investigated. The change of quality components in ganpu tea was determined by high performance liquid chromatography, and the activity of ganpu tea extracts against HepG2 and SGC-7901 colonies was determined by the MTT method. Then, the cell cycle and apoptosis of HepG2 cell line with obvious effect were further analyzed. The results showed that after the processing, the content of polyphenols was 17.22% which was lower than the material tea. Among them, the ester catechins and non-ester catechins were remarkably reduced. Moreover, the increase of tea pigments (theaflavins and thearubigins) was prominent. Ganpu tea had a higher tumor inhibition rate on SGC-7901 and HepG2 cell lines than puer tea and citrus peel, and the IC50 values were up to 176.61 μg/mL and 143.60 μg/mL, respectively. The analysis of cell cycle and apoptosis indicated that ganpu tea induced cell apoptosis through retarding the G0/G1 in HepG2 cells. As a result, the special processing of ganpu tea not only prompted the amelioration of flavor but also improved the accumulation of active substances so that ganpu tea showed better antiproliferative activities in HepG2 and SGC-7901 tumor cell lines.
