Rosa roxburghii Tratt Wine can Improve Glucose Metabolism Disorder in Type 1 Diabetic Rats through Insulin- PI3K Pathway
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Abstract:
To investigate the effect of Rosa roxburghii Tratt wine on the glucose metabolism disorder induced by streptozotocin (STZ) in type 1-diabetic rats, the type 1 diabetic rats model induced by intraperitoneal injection of STZ was established. The rats were randomly divided into blank group, model group, positive group, high, middle and low dose groups, blood glucose is measured every 2 weeks. After 28 days of administration, growth status and relative tissue weight were investigated. The contents of insulin (Ins) in serum were determined by enzyme-linked immunosorbent assay (ELISA). The mRNA levels of PI3K, PKB and GLUT2 in liver, and GLUT4 in skeletal muscle were determined by real time polymerase chain reaction (RT-PCR). The results showed that Rosa roxburghii Tratt wine significantly increase body weight and feed efficiency (p<0.05), significantly reduce fasting blood glucose (21.20%, 36.14% and 8.75%) and glucose tolerance (11.32%, 16.65% and 8.33%), relative tissue weight (p<0.05). when compared with model group. Moreover, Rosa roxburghii Tratt wine significantly increased mRNA relative expressions of PI3K, PKB and GLUT2 in liver and GLUT4 in skeletal muscle. In conclusion, Rosa roxburghii Tratt wine increased the concentration of Ins in serum of diabetic rats and reduced blood glucose levels. Its possible mechanism was that Rosa roxburghii Tratt wine promoted glucose uptake and utilization by activation of the Ins mediated PI3K pathway and translocation of GLUT2/4.
