Ginsenoside F2 Interferes with the Cascade Reactions of Caspase to Inhibit H2O2-induced Apoptosis
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Abstract:
In this study, the anti-apoptotic effect of ginsenoside F2 on H2O2-induced injury in human embryonic kidney HEK-293 cells was evaluated. Using commercial kits, the activities of Caspase-6 and Caspase-9 of apoptotic cells were examined by measured. Western blot tests were conducted to detect the protein expression of the Bcl-2 family (Bcl-2 and Bax) and Caspase family (Cleaved Caspase-3 and Cleaved PARP). After the cells were injured by H2O2, the activity of Caspase increased and the expressions of pro-apoptotic proteins increased. The activities of Caspase-6 and Caspase-9 decreased in a concentration-dependent manner (p<0.05) after the pretreatment of cells with 1.25, 5, 20 μmol/L ginsenoside F2 to injure cells (p<0.05). At the F2 concentration of 20 μmol/L, the activities of Caspase-6 and Caspase-9 decreased by 6.83 U/mg protein and 5.88 U/mg protein., respectively, and the protein expression levels of Cleaved Caspase-3 and Cleaved PARP were significantly lower than those of the injured group (p<0.05). With the increase of F2 concentration, the expression of Cleaved Caspase 3 dropped to 280.90%, 232.46% and 185.26%, respectively. The expressions of Cleaved PARP decreased to 110.36%, 85.85% and 61.95%, respectively, with the increase of F2 concentration. The ratio of Bcl-2/Bax for the high F2 concentration group (73.94%) was significantly higher than that of the injured group (p<0.05). These results showed that ginsenoside F2 can inhibit the activity of apoptotic protein Caspase, and reduce the expression of Bax pro-apoptotic protein and the upstream, midstream and downstream proteins of the Caspase family, through inhibiting H2O2-induced apoptosis via regulating the Caspase cascade reactions.
