Determination of Vitamin K2 (MK-7) in Nutrient Supplements by High Performance Liquid Chromatography-Fluorescence Spectrometry
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Abstract:
To establish a method for the determination of Vitamin K2 (MK-7) in Nutrient Supplements by high performance liquid chromatography-fluorescence detector (HPLC-FLD), the sample was extracted by isopropanol ultrasonic for 30 min, after centrifugation and filtration, 10 μL of the sample solution was injected into the liquid chromatography system. The sample solution was separated by Agilent C18 column (4.6 mm×150 mm, 5 μm) or equivalent column. After the column, it was derived by zinc powder reduction column (4.6 mm×50 mm), so that the Vitamin K2 (MK-7) in the sample had fluorescence characteristics. Methanol (containing 5% tetrahydrofuran, glacial acetic acid 0.03%, zinc acetate 1.0 g/L) was used as mobile phase, the flow rate was 1.0 mL/min, the fluorescence detector was set at λex/λem=243 nm/430 nm. When the sample weight was 1.0 g and the volume was 10 mL, the detection limit of this method was 0.03 μg/g, and the quantitative limit was 0.1 μg/g, MK-7 had a good linearity in the range of 0~2.176 μg/mL, the R2 was 0.9999. The relative standard deviation of the repeatability experiment was 0.97%, and the recovery of the standard was 93.40%. This method has simple operation, good accuracy, and good reproducibility. It can be used for the determination of MK-7 in Nutrient supplements.
