Simultaneous Detection of 7 Benzimidazoles Residues in Carp by Colloidal Gold Immunochromatographic Strip
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
In order to reduce its harm to human, a colloidal gold immunochromatographic strip was established based on monoclonal antibody (mAb) to detect the residue of benzimidazoles in carp. Firstly, the immunogen was prepared using 2-(methoxycarbonylamino)- 3H-benzimidazole-5-carboxylic acid as hapten and coupling protein by activated ester method, mice were immunized and mAb was prepared by cell fusion. Then A colloidal gold immunochromatographic strip was prepared by mAb labelled with synthetic colloidal gold. The 50% inhibitory concentrations (IC50) of albendazole, mebendazole, albendazole s-oxide, albendazole sulfone, fenbendazole flubendazole, oxfendazole were 0.44, 0.16, 3.47, 5.62, 0.62, 0.10, 5.77 ng/mL by ic-ELISA, respectively. The limit of detection (LOD) of albendazole, mebendazole, albendazole s-oxide, albendazole sulfone, fenbendazole, flubendazole, oxfendazole were 4, 2, 50, 100, 10, 1, 100 ng/g in carp samples, respectively. Therefore, the developed method was highly sensitive, rapid, low-cost, free-instrument and applicable for on-site detection of a large numbers of carp samples.
