Identification of Three Listeria Species in Foods based on High Resolution Melting Curve Analysis
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
In this study, a new pair of primers was designed to identify Listeria monocytogenes, Listeria evanescens and Listeria innominata at the same time with iap gene as the target by using high-resolution fusion curve method. The amplification of the other 14 common foodborne pathogens was negative. The detection limit of Listeria monocytogenes and Listeria evanescens was 10 copies, and that of Listeria innominata was 50 copies. In this study, 78 samples were tested by HRM-real time PCR method, GB4789.30-2016 and SN/T 1870-2016 (real-time PCR), and the results of three methods were analyzed statistically. The results showed that there were statistical differences between the detection results of HRM method and national standard method, HRM method and fluorescent PCR method, and there was no statistical difference between the results of national standard method and fluorescent PCR method. The method based on HRM-real time PCR for the detection of three Listeria species in this study is fast, efficient, specific, and low cost. It is suitable for the daily detection and supervision of Listeria species in food.
