Preparation and Antioxidant Activity of Bergamot Essential Oil Liposomes
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Abstract:
Using soybean lecithin and cholesterol as wall materials, bergamot essential oil liposomes were prepared by membrane dispersion and high pressure homogenization. The parameters of preparation and formulation were selected according to the particle size, zeta potential and entrapment efficiency of liposome, and the optimal preparation process was determined. The morphology and stability of liposome were investigated, and its antioxidant activity was also studied. The optimal process parameters were obtained as follows：Homogenization pressure 120 MPa, homogenization times 5 times, phospholipid/cholesterol ratio 5:1, essential oil addition amount 3.5 mg/mL. The particle size of the liposome prepared under these conditions was 183.53 nm, the PDI polydispersity index was 0.147, the zeta potential value was 38.75 mV, encapsulation efficiency was 47.23%, and drug loading was 1.67 mg/mL. The liposomes had good centrifugation stability and storage stability for 30 days at 4 ℃, the retention rate of essential oil was 85.03%, and the scavenging rates of DPPH, ABTS and hydroxyl radicals were 82.58%, 78.02% and 70.89%, respectively. Conclusion: Under the conditions of homogenization pressure of 120 MPa, homogenization times of 5, ratio of phospholipid to cholesterol of 5:1, and the amount of essential oil of 3.5 mg/mL, the liposome prepared has moderate particle size, good stability and good antioxidant activity.
