Enzymatic Production and Lactobacillus Proliferation of Galactooligosaccharidesby β-Galactosidase from Lactobacillus plantarum in vitro
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Abstract:
Enzymatic synthesis of prebiotic galactooligosaccharides (GOS) using the β-galactosidases produced from Lactobacillus plantarum was investigated. In this work, the effects of synthetic conditions including concentration of lactose substrate, reaction temperature, pH of system, enzyme concentration on the formation and the monitoring of the proliferation of lactic acid bacteria in vitro, total output and optimum conditions of different pure components of GOS were evaluated. Its probiotic activity was characterized. The results showed that the major reaction conditions were concentration of substrate, temperature, whereas pH and concentration of enzyme were secondary conditions. The optimal conditions for the total output was listed as followed: 45°C, substrate concentration of 400 g/L, pH 7.0, enzyme concentration of 10 U/mL, time of 10 h. Under these conditions, the total GOS output was 177.26 g/L, accounted for 44.31% (m/V) in the reaction system, including allo-lactose, 6′-galactobiose, 6′-GOS and 3′-GOS, they were 63.22 g/L, 15.32 g/L, 72.21 g/L and 25.11 g/L, respectively. For the proliferation of lactic acid bacteria in vitro, the new GOS was more remarkable by comparison to the commercialized GOS, as exhibited in the same amount of microbodies at relatively low concentration of GOS and the obvious advantage of maximum specific growth rate.
