Inhibition Activity of Oral Cancer KB Cell of Chondroitin Sulfate from Different Sources
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Abstract:
Chondroitin sulfate was extracted from the sturgeon cartilage and chicken cartilage using the alkaline extraction-enzymatic-alcohol precipitation process, and purified by Sephadex G-50 gel chromatography and Sephacryl S-300 HR gel chromatography. The molecular mass and purity of the obtained chondroitin sulfate were determined by HPGPC. The effect of chondroitin sulfate on the proliferation of oral cancer KB cells was detected by the MTT assay, and the apoptosis of oral cancer KB cells was examined by flow cytometry. The results showed that the yield of crude chondroitin sulfate from sturgeon cartilage and chicken cartilage was 23.80% and 25.23%, respectively. HPGPC analysis showed that the purified chondrocytes from sturgeon cartilage and chicken cartilage were highly pure polysaccharides, with the average molecular weight of the chondroitin sulfate in sturgeon cartilage higher than that in chicken cartilage. The inhibitory effects of crude and purified chondroitin sulfate on oral cancer KB cells were studied in vitro. The results showed that the IC50 values were 80.18 μg/mL and 58.78 μg/mL, respectively, for the crude and purified chondroitin sulfate from sturgeon cartilage, and 68.55 μg/mL and 53.71 μg/mL, respectively, for the crude and purified chondroitin sulfate from chicken cartilage. Flow cytometry showed that chondroitin sulfate could induce the apoptosis of KB cells, and the apoptosis rate was dependent on the concentration of the chondroitin sulfate. The apoptosis rate induced by the chondroitin sulfate in chicken cartilage was higher than that from sturgeon cartilage.
