Chicken Enzymatic Hydrolysates Improved Anti-fatigue and Antioxidant Activities in Mice
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Abstract:
This study investigated the anti-fatigue and anti-oxidation effects of chicken enzymatic hydrolysates EP1 and EP2. Normal saline and chicken homogenate [protein dose 200 mg/(kg·bw·d)] were used as the control group, and two doses of EP1 and EP2, 40 mg/(kg·bw·d) and 200 mg/(kg·bw·d), were administered by gavage into the mice. After 28 days of administration, the exhausted swimming time, and the serum urea nitrogen content, serum lactic acid content and liver glycogen content in the mice after 30 min of swimming were measured. The activities of SOD (superoxide dismutase), GSH-Px. (glutathione peroxidase) and CAT (catalase) in mouse serum were also analyzed. The results showed that chicken hydrolysates could effectively prolong the exhausted swimming time of mice (the SC group 766 s; the H-EP1 group 2307 s). After 30 min of swimming, the contents of serum lactic acid (the SC group 10.06 mmol/L; both the H-EP1 and H-EP2 groups 6.66 mmol/L) and serum urea nitrogen (the SC group 19.72 mmol/L; the H-EP1 group 13.34 mmol/L) decreased significantly, whilst the content of liver glycogen (the SC group 5.11 mg/g; the H-EP2 group 8.35 mg/g) as well as the activities of serum SOD, GSH-Px. and CAT antioxidant enzymes increased significantly. The research results suggest that chicken enzymatic hydrolysates EP1 and EP2 could significantly extend the exhaustion swimming time of mice, reduced the consumption of liver glycogen and the production and deposition of lactic acid in mice after intense exercise, promoted protein metabolism, removed effectively the serum urea nitrogen in mice, increased the antioxidant activities of serum SOD (from 89.08 to160.13 U/mL), CAT (from 0.25 to 0.29 U/mL) and GSH-Px. (from 257.36 to 597.08 U/mL) in mice, enhance the free radical scavenging ability of mice, and improved the exercise status of mice. These changes ensure that the mice subjected to intense exercise remained in the mode of aerobic exercise, so as to reduce the body’s fatigue after intense exercise, with significant anti-fatigue and anti-oxidation effects.
