Antibiotic Resistance of Lactic Acid Bacteria Strains Isolated from Intestinal Microflora of Penaeus vannamei
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Abstract:
In this work, P. vannamei was used as the research object. The 26 strains of lactic acid bacteria were isolated from the intestine and classified into 4 species: L. lactis subsp. Lactis, L. taiwanensis, L. garvieae and L. lactis. Seven representative lactic acid bacteria were selected to study their resistance to antibiotics from the genotype and phenotype based on the methord of PCR as well as the antibiotic selective plate coating method. The results showed that 7 strains of lactic acid bacteria had similar drug resistance spectrum, indicating resistance to tetracycline, sulfapyridine, acetylspiramycin (inhibition rate < 50.00%) while being sensitive to gold mold, oxytetracycline, chlortetracycline sulfate, chloramphenicol and erythromycin (inhibition rate 100%). More than 3 ARGs were detected in all strains with the detection rate of ARGs were as the follows: sulfonamides (92.86%) > tetracyclines (53.66%) > quinolones (23.81%) > aminoglycosides (4.76%) > chloramphenicol = macrolides (0.00%). The ARGs genotype did not exactly match the antibiotic phenotype of the strain.
