Extraction Optimization of Total Triterpenoids from Artemisia argyi and Its Antibacterial Activity
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Abstract:
The effects of solvent type, solvent concentration, extraction temperature, liquid-solid ratio and ultrasonic time on the degree of total triterpenoids yield were investigated using Artemisia argyi from Enshi. The four factors were further optimized using response surface methodology based on a three-level, four-variable Box-Behnken design. By analyzing the response surface plots, the optimal levels for total triterpenoids from Artemisia argyi were found. And the antibacterial activity of total triterpenoids was explored through testing the minimum inhibitory concentration (MIC) of different components on 7 test bacteria by the double dilution method. Results showed that the optimal process parameters of ultrasound extraction for total triterpenoids from Artemisia argyi were found to be ethanol concentration of 72%, extraction temperature of 73 ℃, liquid- solid ratio of 20:1 (mL/g) and ultrasonic time of 42 min. Under these conditions, the actual total triterpenoids yield was 6.51%, and the ultrasonic time and temperature were significant to the total triterpenoids from Artemisia argyi. The total triterpenoids from Artemisia argyi had certain inhibitory effect on 7 common pathogenic bacteria, especially had inhibitory effects on Staphylococcus aureus and Streptococcus pneumoniae, the minimum inhibitory concentration (MIC) was 3.00 mg/mL. The process was simple and reasonable, while the extraction had a strong broad-spectrum antibacterial effect which provided a scientific basis for deeply research total triterpenoids from Artemisia argyi.
