Inhibitory Effect of Selenium Rich Protein of Ganoderma lucidum on HepG2 Cells
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Abstract:
To explore the effect of Ganoderma selenium rich protein on HepG2 cell proliferation, apoptosis, cycle distribution, invasion and migration, human hepatocellular carcinoma HepG2 cells were selected and divided into blank group, drug control group, selenium-free chiroderma lucidum protein group, low, medium and high concentration groups. Biological behaviors of cells and expressions of bcl-2, Bax and caspase3 in the six groups were detected. The results showed that the cell proliferation rate of high concentration group was lower than that of other groups, and the apoptosis rate was higher than that of other groups. The number of cell invasion and migration in high concentration group was 28.21 and 29.66, respectively, lower than that in other groups (p<0.05). The expression of Bcl-2 and Caspase-3 in the high concentration group was 0.98 and 1.01, respectively, lower than that in other groups; the expression of Bax was 1.03, higher than that in other groups (p<0.05). The intervention with Ganoderma selenium rich protein can affect the proliferation and apoptosis of hepatoma cells, block cell cycle, inhibit invasion and migration, regulate the expression of Bcl-2, Bax and Caspase-3, which shows that Ganoderma selenium rich protein can regulate the proliferation, apoptosis, invasion and migration of hepatoma HepG2 cells, inhibit the development and diffusion of hepatoma HepG2 cells, and provide certain rationale for the clinical treatment of hepatoma.
