Antioxidant Activity and Anti-inflammatory Effect on Acute Inflammatory Mice of Resveratrol-loaded Zein-pectin Nanoparticles
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Abstract:
The anti-oxidative ability after simulated digestion in vitro and the anti-inflammatory effects of LPS-induced mice of resveratrol-loaded zein-pectin nanoparticles (En-RES) were studied. An antisolvent/electrostatic deposition method was used to prepare En-RES. The H2O2-induced oxidative stress model was treated with En-RES digestive fluid after simulated gastrointestinal digestion, and the contents of malondialdehyde (MDA) and antioxidant enzymes were measured. The 72 mice were randomly divided into the normal group, lipopolysaccharide (LPS) model group, free resveratrol groups (low, medium and high concentrations) and En-RES groups (low, medium and high concentrations). The plasmas were collected for determination of interleukin-1α (IL-1α), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10) and nitric oxide (NO) level. The results showed that, when compared with free resveratrol (RES) digestion fluid treated oxidative stress reacted HepG2 cells, the intracellular MDA content of the cells treated with En-RES digestion fluid decreased by 49.07%, and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities were increased by 35.27%, 67.22% and 13.15%, respectively (p<0.05). In the mouse acute inflammation experiment, compared with the RES high concentration treatment group, the plasma levels of IL-1α, IL-1β, IL-6 and NO in the high concentration group of En-RES decreased by 62.73%, 44.91%, 52.86% and 55.47%, respectively, and IL-10 levels were increased by 66.49% (p<0.05). This study showed that resveratrol-loaded zein-pectin nanoparticles could improve the antioxidant capacity in vitro and anti-inflammatory capacity in vivo of resveratrol.
