Protective Effects of Oyster Oligopeptide against H2O2-induced Oxidative Damage in Human L02 Cells
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Abstract:
To study the scavenging capacity of free radicals and protective effect of oyster oligopeptide against hydrogen peroxide (H2O2)-induced oxidative damage in human liver L02 cells were determined. A H2O2-induced oxidative injury model was established to analyze the influence of oyster oligopeptide on the survival rate of L02 cells, level of reactive oxygen species (ROS), and contents of antioxidant enzymes (superoxide dismutase/SOD and glutathione/GSH). Results showed that IC50 of oyster oligopeptide for hydroxyl free radical was 0.38 mg/mL. The proliferation rate of L02 cells treated with oyster oligopeptide at 2 mg / mL was still 123.98%. The levels of SOD and GHS of the model group treated with 400 μM H2O2 decreased by nearly 40% and 64.87%, respectively, while the level of ROS increased by a factor of two. After the addition of oyster oligopeptide at different concentrations, the level of SOD in the middle dosage group was almost reversed to the normal level, while the activity of GSH increasing by 118.89% and the level of ROS decreasing to 62.43% of that for the model group. These results indicated that oyster oligopeptide was nontoxic to L02 cell, and could decrease the level of ROS and enhance the contents of antioxidant enzyme SOD and GSH in the oxidative stressed L02 cells, providing protective effects on cells. Therefore, oyster oligopeptide was capable of protecting human L02 cells against H2O2-induced oxidative injury, possibly through scavenging free radicals, protecting cellular antioxidant enzymes, and inhibiting the excessive accumulation of ROS.
