Abstract:In order to shorten the propagating time of Listeria monocytogenes and improve the detection efficiency, yeast (ATCC 9763) was added to LB1 medium of Listeria monocytogenes as a growth promoter. The results showed that the growth rate of Listeria monocytogenes could be increased about 100% by adding appropriate amount of yeast. Moreover, the promotion effect was related to the inoculation amount of yeast, dissolved oxygen in the medium and the culture mode. According to the analysis of the growth characteristics of yeast, maybe the degradation of saccharides and other nutrients by yeasts improved the growth environment, increasing the growth rate of Listeria monocytogenes. This result laid a good foundation for shortening the pre-enrichment time and improving the detection efficiency of Listeria monocytogenes. On the other hand, it also suggested that more attention to the food safety risk of fermented food contaminated by Listeria monocytogenes should be paid.
