False-positive Interfering Substance to the Measurement of Sodium Saccharin in Ice Cream Using UPLC-PDA/Q-TOF
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Abstract:
The false-positives to the detection of sodium saccharin in ice creams (according to the National Food Safety Standard GB 5009.28-2016 issued by the National Health Commission of the People's Republic of China) were examined, based on the ultra high performance liquid chromatography-photo diode array detector/quadrupole-time of flight tandem mass spectrometry (UPLC-PDA/Q-TOF) and the high resolution mass spectrometry-nontarget data acquisition-automatic spectrum peak identification strategy. It could be inferred that the positive and negative molecular ion mass-to-charge ratios of the interfering compound were 180.0626 and 178.0506 respectively, through UPLC-PDA positioning, MSE mode for full-scale online acquisition of mass spectrometric samples, UNIFI data processing software comprehensive analysis of interferences; The compound was speculated to be hippuric acid based on further structural analysis. MSMS mode along with the standard substance were used to verify the identification results, and it was found that the variation in retention time between hippuric acid and the interfering substance was 0.02 min, with the same mass-to-charge ratios of molecular ions and fragment ions (178.0509 and 134.0623, respectively), and the same chromatographic and mass spectrometric behaviors. Thus, the interfering compound was identified as hippuric acid. This work provided certain theoretical foundation and technical support for the detection of sodium saccharin and the qualitative study on unknown compounds, therefore, is of great significance for relevant dairy industries to control product quality.
