Application of High-throughput Sequencing and Gene Barcode Technology in Species Identification of Animal-derived Products
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Abstract:
This study used high-throughput sequencing and DNA barcoding technology to make an exploratory study on the identification method of animal-derived components in biological products. Nucleic acid was extracted from common mammals, avian, fish species and a variety of mixed samples. PCR technology was used to amplify the 354 bp detection site of the mitochondrial gene 16S rRNA region. Illumina Miseq sequencing technology was used to obtain all sequence information, and the species composition in the samples was compared with GenBank database. The results showed that all the components of animal origin in the mixed samples were correctly identified; the minimum detection limit of nucleic acid of goose and duck was 0.5 ng/μL; two labels of commercial products of animal origin were found to be inconsistent after testing. The detection method achieved a one-time acquisition of mixed DNA fragment sequence information in a high-throughput sequencing and result comparison analysis experimental cycle. The test results are accurate and applicable in a wide range, and it is expected to provide technical support for animal product identification compliance inspection and anti-smuggling.
