Molecular Typing of Foodborne Campylobacter jejuni and Analysis of Its Genetic Diversity
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Abstract:
In order to understand the genetic diversity of food-borne Campylobacter jejuni isolated in 2012~2014 from different cities of China, multilocus sequencing typing (MLST) was used for molecular typing of the 33 isolated strains. In the study, the housekeeping genes of 33 strains were amplified by PCR using 7 different pairs of primers, and the amplified products were identified by gel electrophoresis and sequenced. The sequencing results were compared with the Campylobacter database of PubMLST to obtain the corresponding sequence types, and the data of the new sequence type were submitted to the curator. A phylogenetic tree and minimum spanning tree were constructed according to MLST data. The results showed that 33 food-borne C. jejuni strains could be divided into 27 STs, of which 14 were new STs. They could form 11 clonal complexes, with the dominant clonal complexes being CC22 and CC45, and some CC-types were found clinically. There were 91 nucleotide polymorphism sites, with some alleles being recombined. These results demonstrate the genetic diversity of the strains isolated between 2012 and 2014 and their potential pathogen risks.
