Antioxidant Activity and Combined Antioxidant Effect of Phenolic Acids and Glutathione
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Abstract:
In order to investigate the antioxidant activities of glutathione and six phenolic acids (syringic acid, vanilic acid, gallic acid, caffeic acid, sinapic acid, ferulic acid) alone and in combination, ABTS+· scavenging ability, DPPH· scavenging ability, reducing power and oxygen free radical absorption capacity were used to evaluate their antioxidant activities, and the synergistic effect (SE) was used to analyze whether they have synergistic antioxidant. The results showed that glutathione had antagonistic effects with syringic acid and vanillic acid in the four antioxidant experiments, and had obvious synergistic effects with gallic acid only in reducing power experiment (p<0.05). There was no synergistic effect between glutathione and ferulic acid, but only synergistic effect with sinapic acid in DPPH· scavenging experiment. However, glutathione and caffeic acid showed significant synergistic antioxidant activity in ABTS, DPPH free radical scavenging experiment and reducing power experiment and its SE were 1.21, 2.20 and 1.29, respectively, which was the most potential synergistic antioxidant combination.