Apoptosis of Sophora japonica Extraction on Hepatoma Rats
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Abstract:
To investigate the interventional effect and working mechanism of Sophora japonica ethanolic extract on apoptosis of hepatoma in rats. Fifty SD rats were selected, with 10 for the normal group and 40 set up to have hepatic carcinoma (which were divided into the model group, drug control group, low- and high-dose intervention group). The cell cycle distribution, proliferation and apoptosis of rats in each group were examined, and the expressions of PTEN, p-akt, mTOR, bcl-2, PI3K and Akt were analyzed. The results showed that the proportion of cells at G1 phase, apoptosis rate and PTEN relative expression level for the high-dose intervention group were 54.72%, 45.34% and 0.73, respectively, which were all higher than those of the model group, drug control group and low-dose intervention group (p < 0.05). The proliferation rate, the relative expression levels of p-Akt, mTOR, Bcl-2, PI3K and Akt were 13.33%, 1.33, 1.34, 1.31, 1.32 and 1.35, respectively, which were all lower than those of the model group, drug control group and low-dose intervention group (p<0.05). The intervention with sophora japonica ethanolic extract significantly regulated the cell proliferation and apoptosis of carcinoma tissues as well as the expression of PTEN, p-Akt, mTOR and the Bcl-2, PI3K and Akt in rats with liver cancer. These results indicate that, Sophora japonica ethanolic extract can block the cell cycle distribution, inhibit cell proliferation and promote apoptosis of carcinoma tissues in rats. Such capacities depend on the concentration of sophora japonica ethanolic extract, which provides certain reference value for clinical treatment of liver cancer symptoms.
