Anti-aging Effects of Phloretin on Female Drosophila melanogaster
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Abstract:
The anti-aging effects of phloretin on female Drosophila melanogaster and its potential mechanism were explored. By supplementing serially diluted phloretin (1.15 mM, 2.3 mM, 4.6 mM) to female Drosophila melanogaste, the effects of phloretin on survival rate, crawling ability, stress resistance, intestinal barrier function and microbial colonies, number of intestinal progenitor cells, activity of endogenous antioxidant enzymes of SOD, CAT and GSH-PX and content of lipid peroxidation product MDA, related antioxidant genes SOD2, CAT, MTH and foxo mRNA expression level were investigated. The Results showed that female Drosophila melanogaster treated with 4.6 mM phloretin was markedly extended the mean lifespan by 23.98%, crawling ability increased by 44.96% at 30 days, lifespan after H2O2 and SDS injury increased by 24.35% and 23.94%, respectively, the number of intestinal precursor cells reduced by 15.59%, the number of LMRS, ENT, ACE, NR in the gut of Drosophila melanogaster reduced by 23.73%, 33.33%, 43.24% and 27.50%, respectively, at 40 days of age. The activity of Mn-SOD, Cu/Zn-SOD, CAT and GSH-PX increased by 17.85%, 31.03%, 32.07% and 25.57%, respectively; the content of MDA decreased by 23.55%, the mRNA expression of foxo, CAT and SOD2 were increased by 67.21%, 32.54% and 36.27%, respectively, while the mRNA expression of MTH was decreased by 27.16%. Phloretin can improve the antioxidant enzymes activity and antioxidant gene expression levels, enhance the stress resistance, promote intestinal homeostasis and prolong the lifespan of female Drosophila melanogaster.
