Bacterial Diversity Analysis of Infant Food Based on 16S rRNA High-throughput Sequencing
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Abstract:
In order to investigate the bacterial community structure in infant formula milk and rice flour, and provide theoretical basis for the quality control and supervision of infant food production, the 16S rRNA V4-V5 regions of bacteria from 14 infant food samples were sequenced based on ion torrent PGM platform. A total of 13,805 different OTUs were obtained by sequencing analysis, belonging to 2 kindoms, 10 phyla, 20 classes, 50 orders, 84 families and 144 genera. The results showed that the bacterial community diversity of the infant milk powder samples was higher than that of the rice flour, and the difference within group of milk powder was smaller than that of rice flour. The absolute dominant flora in goat milk powder belonged to Fimicutes (65.58%) and Proteobacteria (16.01%) on phylum level, Streptococcus (24.86%) and Lactococcus (21.44%) on genus level. While, Cyanobacteria (41.79%) was the most dominant in rice flour samples, followed by Fimicutes (30.99%) and Proteobacteria (24.48%), which might be related to the different raw materials of two products. In addition, foodborne pathogens such as Salmonella (8.75%) and Listeria (3.61%) existed in most of the rice flour samples, and the difference of abundance was significant (p<0.05) between the milk powder and rice flour samples, which indicated that infant rice flour products might have potential food safety risks. Therefore, the enterprises and food supervision department should pay more attention to the quality control of infant rice flour production.
