Evaluation on Antioxidant and Anti-inflammation in Vitro of Extracts of Three Solanum Nigrum L. Berries
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Abstract:
Solanum nigrum L. berries have been widely used for anti-pyretic and diuretic in China. Antioxidant and anti-inflammation activities in vitro of extracts of Solanum nigrum Linnaeus (SNL), Solanum diphyllum Linnaeus (SDL) and Solanum alatum Moench (SAM) berry which were prepared by petroleum ether (PE), ethyl acetate (EtoAc) and n-butanol (n-BuOH). The antioxidant activites were evaluated by FRAP and DPPH· scavenging assays. Vitamin C was used as positive control. The results showed that DPPH· scavenging IC50 and FRAP values of SNL-EtoAc extract were 119.43±1.19 μg/mL and 2.674±0.071 mM FeSO4/L, respectively, indicating that it had the highest antioxidant activity. The anti-inflammation effect of different extracts was determined by hyaluronidase and lipoxygenase inhibition. SAM-PE extract had the highest anti-inflammation activity, which IC50 were 810.67±79.02 and 781.28±28.01 μg/ml, respectively. Diclofenac sodium and 4,4'-(2,3-Dimethyltetramethylene) dipyrocatechol were used as anti-inflammatory positive control. SNL had the highest content of total phenols (27.16±0.14 mg/g). SAM had the highest content of total flavonoids (18.59±0.14 mg/g). The results provided a theoretical supporting for developing natural antioxidant and anti-inflammatory resources.
