Simulated Gastrointestinal Digestion Increase the in Vitro Antioxidant Activity of Cordyceps militaris Proteins
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Abstract:
Physicochemical properties and antioxidant activity of Cordyceps militaris proteins and their simulated gastrointestinal digestive products were investigated. Molecular weight distribution and amino acid composition of the proteins and digestive products were measured by SDS-PAGE and acid hydrolysis method, respectively. Antioxidant activity of Cordyceps militaris proteins and their simulated gastrointestinal digestive products was evaluated by in vitro chemical methods. Oxidative damaged PC12 cell model induced by H2O2 was established. After treatment with the digestive products, cell viability was measured by CCK-8. The results showed that after digestion, the molecular weight distribution shifted from 10~120 ku to 8~15 ku; the IC50 values of DPPH clearance rate of Cordyceps militaris proteins and their digestive products were 1.55 and 1.06 mg/mL, respectively. The IC50 values of Fe2+ chelating power were 1.32 and 0.18 mg/mL, respectively. The ORAC values were 467.27±46.53 and 948.80±24.02 μmol TE/g respectively when the sample concentration was at 0.3 mg/mL. The cell viability of PC12 cells injured by H2O2 was significant improved (p<0.05) after pre-treated with 100 μg/mL digestive products. Both of Cordyceps militaris proteins and their digestive products possess high lipid per-oxidation inhibition ability and low ferric reducing power. Thus, more peptides with lower molecular weight were generated after simulating gastrointestinal digestion of Cordyceps militaris proteins. Of note, the antioxidant activity improved significantly. It is therefore plausible to further purify and characterize the antioxidant peptides in simulated gastrointestinal digestive products of Cordyceps militaris proteins.
